Gao et al.: Massive risks with COVID gene injection boosting (in a mice model) "Extended SARS-CoV-2 RBD booster vaccination induces humoral and cellular immune tolerance in mice"; aka, immune failure
by Paul Alexander
Repeated immunizations impaired serum neutralization activity & suppressed formation of germinal center & inhibited activation of CD8+T cells; Did mice exhibit immune tolerance or exhaustion?
The findings in this paper (mice model) supports what has accumulated across the last 2 years (especially with human epidemiological data) with the COVID vaccine roll-out which is that there is negative effectiveness with rapid waning vaccinal immunity, and as per Fantini et al. and Liu et al. (see my prior substack), there is substantially increased infectiousness of the virus to the vaccinated.
The vaccine appears to give the virus properties it prior did not have and the evidence is clear that the more shots (boosters) on gets, the more one gets re-infected, hospitalized, and with risk of death. Similarly as per this paper, the more the immune system is impaired. This was seen in a recent Shrestha et al. publication (Cleveland clinic) in a prior substack I wrote whereby the more shots one took, the more the risk of infection and a clear dose-response was seen:
Now this present mice model study by Goa et al. had some key findings:
-Extended immunization inhibited the production of RBD-specific memory B cells
-Extended immunization reduced serum neutralizing antibody responses
-Extended immunization suppressed the formation of the germinal center
-Extended immunization inhibited the activation of CD4+T cell immune responses
-Extended immunization inhibited CD8+ T cell-mediated immune response
Was this immune tolerance? Was this immune exhaustion in the mice model?
Researchers reported: ‘We used a rodent animal model instead of primates in this study. Although the actual kinetics of immune reactivity between mice and humans is not fully understood, the Balb/c mice model has been shown to share profound similarities with humans in response to SARS-CoV-2 infections (Halfmann et al., 2022).’
Key expanded findings statements made by the researchers (note, this is in a mice model and not in a human model but we can extrapolate when such humanized mice are used, as they are altered to mimic the respiratory and immunological response in humans):
i)‘Our findings demonstrate potential risks with the continuous use of SARS-CoV-2 vaccine boosters, providing immediate implications for the global COVID-19 vaccination enhancement strategies.
ii)Whether such re-establishment of vaccine-induced immune response could be repeated by continued application of boosters is being questioned, yet largely unknown at present. Here, we compared the effects of repeated RBD vaccine boosters with a conventional immunization course to those with an extended vaccination strategy, in a Balb/c mice model.
iii)We found that the protective effects from the humoral immunity and cellular immunity established by the conventional immunization were both profoundly impaired during the extended vaccination course. Specifically, extended vaccination not only fully impaired the amount and the neutralizing efficacy of serum RBD-specific antibodies, but also shortened the long-term humoral memory.
iv)This is associated with immune tolerance in germinal center response, along with decreased numbers of spleen germinal center B and Tfh cells. Moreover, we demonstrated that extended immunization reduced the functional responses of CD4+ and CD8+T cells, restrained the population of memory T cells, and up-regulated the expression of PD-1 and LAG-3 in Te sub-type cells.
v)An increased percentile of Treg cells was also observed, accompanied by significant elevation of IL-10 production. Together, we provided crucial evidence that repetitive administration of RBD booster vaccines may negatively impact the immune response established by a conventional vaccination course and promote adaptive immune tolerance.’
vi)Continued vaccination promoted the formation of a prominent adaptive immune tolerance and profoundly impaired the established immune response with the conventional course, evidenced by significant reductions in antigen specific antibody and T cell response, a loss of immune memory and form of immunosuppression micro-environment.
The researchers Gao et al. could not be more clearer than that. The repeated booster is subverting the immune system and it fails, using this mice model. And vaccine Taliban cannot come out saying ‘well, that’s in a mice model so not to be really extrapolated to humans’ when the same COVIDian Taliban lockdown lunatics waging their informational and media jihad on us, on our minds and senses 3 years now, lying to us and deceiving us with their lies, used the same 8 mice model to approve the same bivalent booster. They did not even use human data and FDA gave the EUA with the rodent data (8 mice and the mice themselves got sick from the vaccine) and at that time the only human data used different sub-variants. That is the insanity we live with these vaccine Taliban adherents. I have to call them Taliban (Pfizer and Moderna), I can find no better descriptive.
‘Continued vaccination promoted the formation of a prominent adaptive immune tolerance and profoundly impaired the established immune response with the conventional course, evidenced by significant reductions in antigen specific antibody and T cell response, a loss of immune memory and form of immunosuppression micro-environment. Our findings demonstrated the potential risks associated with an extended vaccine booster course of SARS-CoV-2 vaccination, with immediate implications for the strategic use of homology booster vaccines.’
They ‘compared the humoral and cellular immune responses of an extended course of recombinant receptor binding domain (RBD) vaccine boosters with those from conventional immunization strategy in a Balb/c mice model. Multiple vaccine boosters after the conventional vaccination course significantly decreased RBD-specific antibody titers and serum neutralizing efficacy against the Delta and Omicron variants, and profoundly impaired CD4+ and CD8+T cell activation and increased PD-1 and LAG-3 expressions in these T cells. Mechanistically, we confirmed that extended vaccination with RBD boosters overturned the protective immune memories by promoting adaptive immune tolerance. Our findings demonstrate potential risks with the continuous use of SARS-CoV-2 vaccine boosters, providing immediate implications for the global COVID-19 vaccination enhancement strategies.’