Is there nuclear (nucleus) translocation of COVID spike mRNA & protein? Sattar et al. says yes & reports one of the novel sequence insertions resides at the S1/S2 boundary of Spike (S) protein and
by Paul Alexander
constitutes functional nuclear localization signal motif "PRRARSV", which may supersede importance of previously proposed furin cleavage site "RRAR"; did Malone know mRNA translocates to the nucleus?
Did Robert Malone know (and Kariko etc.) that his mRNA technology translocates to the nucleus with possible pathogenic consequences as Sattar et al. found? Did Kariko and Weissman know? Did others in the mRNA technology world know? When did Malone know it and if he knew it before the vaccine was rolled out, why was he silent? We now have clear evidence that it reverse transcribes (with possibly devastating consequences to the human genome/DNA and humanity as we have no idea how this will unfold for those mRNA technology ‘inventors’ failed to tell us about this and we await his/their explanation what he knew and when he knew this too.
‘The spike (S) protein appears to be a major pathogenic factor that contributes to the unique pathogenesis of SARS-CoV-2. Although the S protein is a surface transmembrane type 1 glycoprotein, it has been predicted to be translocated into the nucleus due to the novel nuclear localization signal (NLS) "PRRARSV", which is absent from the S protein of other coronaviruses. Indeed, S proteins translocate into the nucleus in SARS-CoV-2-infected cells. To our surprise, S mRNAs also translocate into the nucleus. S mRNA colocalizes with S protein, aiding the nuclear translocation of S mRNA. While nuclear translocation of nucleoprotein (N) has been shown in many coronaviruses, the nuclear translocation of both S mRNA and S protein reveals a novel pathogenic feature of SARS-CoV-2.
Author summary: One of the novel sequence insertions resides at the S1/S2 boundary of Spike (S) protein and constitutes a functional nuclear localization signal (NLS) motif "PRRARSV", which may supersede the importance of previously proposed polybasic furin cleavage site "RRAR". Indeed, S protein's NLS-driven nuclear translocation and its possible role in S mRNA's nuclear translocation reveal a novel pathogenic feature of SARS-CoV-2.’