Post COVID vaccination: "SARS-CoV-2 Spike (S 1) Protein Persistence in SARS-CoV-2 Negative Post-Vaccination Individuals with Long COVID/ PASC-Like Symptoms"; elevations of sCD40L, CCL5, IL-6, and IL-8
by Paul Alexander
Vaccinees have markers of platelet activation & pro-inflammatory cytokine production which may be driven by persistence of SARS-CoV-2 S 1 protein persistence in intermediate & non-classical monocytes
Bottom line is this: we are finding spike in the blood etc. 15 months post vaccine (publication to follow). The spike (synthetic) is from the COVID vaccine and we were lied to by the manufacturers and those involved who said it will not stay in the system and this would be one off etc. This study is indicating that those with long-COVID have persistent spike post-vaccination and show aberrant symptoms e.g. markers of platelet activation and pro-inflammatory cytokine production. In other words, the persistence of spike long-term and potential translation of spike from mRNA long-term in the vaccinated person, may account for the symptoms of long-COVID, at least in part. Further urgent study is needed to clarify these findings by Patterson et al.
We sought to determine the immunologic abnormalities in patients following SARS-CoV-2 vaccines who experience post-acute sequelae of COVID-19 (PASC)-like symptoms > 4 weeks post vaccination. In addition, we investigated whether the potential etiology was similar to PASC.
We enrolled 50 post-vaccination individuals who experience PASC-like symptoms, 10 healthy individuals, and 35 individuals post-vaccination without symptoms. We performed multiplex cytokine/chemokine profiling with machine learning as well as SARS-CoV-2 S1 protein detection on monocyte subsets using flow cytometry and mass spectrometry.
We determined that post-vaccination individuals with PASC-like symptoms had similar symptoms to PASC patients. When analyzing their immune profile, post-vaccination individuals had statistically significant elevations of sCD40L, CCL5, IL-6, and IL-8. SARS-CoV-2 S1 and S2 protein were detected in CD16 + monocytes using flow cytometry and mass spectrometry on sorted cells.
Post-vaccination individuals with PASC-like symptoms exhibit markers of platelet activation and pro-inflammatory cytokine production which may be driven by the persistence of SARS-CoV-2 S1 protein persistence in intermediate and non-classical monocytes.’